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Array type dissolved oxygen sensor and measurement system for simultaneous measurement of cellular respiration level

By: Pak, Y.K.; Ahn, S.; Chang, J.-H.; Park, J.; Pak, J.J.; Han, S.;

2009 / IEEE / 978-1-4244-4190-7

Description

This item was taken from the IEEE Conference ' Array type dissolved oxygen sensor and measurement system for simultaneous measurement of cellular respiration level ' This paper describes a miniaturized oxygen sensor array integrated with heaters and temperature sensors and a LabVIEW-based measurement system which exactly can measure a cellular respiration level in a solution containing cells simultaneously. The designed oxygen sensor consists of Clark-type sensors, heaters, and temperature sensors of 2 �3 array type on the substrate. 18�m PDMS was used for a permeable membrane of Clark-type sensor. The 90% response time was about 10 sec from full-oxygen (air-saturated) state to zero-oxygen (0.1M Na2SO3) state. The fabricated sensor showed good reproducibility for 1 hour with 3.15nA standard deviation of 108.2nA mean in the full-oxygen state and 0.14nA standard deviation of 1.33nA mean in the zero-oxygen state. Also, the fabricated sensor showed a good linearity with a correlation coefficient of 0.997. The LabVIEW-based measurement system composed to voltage supply module, amplifier module, DMM module, and temperature sensing module. In order to measure the cellular respiration level, the measurement program using LabVIEW was made. The ratio of uncoupled cellular oxygen consumption rate per coupled cellular oxygen consumption rate obtained by the fabricated sensors showed 1.96 which is almost the same as the value obtained from a commercial Oxygen-2K.