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A PDMS Planar Patch-Clamp Array Chip with Poly (Ethylene Glycol)/SU-8 based Cell-Patch Interface

By: Yu, J.J.; Liu, Z.B.; Xu, B.J.; Yang, M.; Lee, Y.K.;

2009 / IEEE / 978-1-4244-2977-6

Description

This item was taken from the IEEE Conference ' A PDMS Planar Patch-Clamp Array Chip with Poly (Ethylene Glycol)/SU-8 based Cell-Patch Interface ' This paper presents a new PDMS planar patch-clamp array chip integrated with microfluidic units and a poly (ethylene glycol)/SU-8 mixture based cell-patch interface for whole cell recordings. In our experiments, the PEG/SU-8 mixture with the weight ratio of 1:1 has been proven to have improved properties to fabricate perfect apertures compared with PDMS for cell-patch interface, such as large Young's modulus and good hydrophilic surface properties. The integrated chip was tested to trap 20¿m polystyrene microsphere onto 2¿m aperture by a negative pressure, and the variation of impedance spectra in trapping process demonstrated that the excellent sealing resistance has been achieved. Our present results indicate the potential of a planar patch-clamp array chip system with PEG/SU-8 base cell-patch interface for high-throughput whole cell recording.