Your Search Results

Use this resource - and many more! - in your textbook!

AcademicPub holds over eight million pieces of educational content for you to mix-and-match your way.

Experience the freedom of customizing your course pack with AcademicPub!
Not an educator but still interested in using this content? No problem! Visit our provider's page to contact the publisher and get permission directly.

Use of silicon and GaAs pixel detectors for digital autoradiography

By: Amendolia, S.R.; Stefanini, A.; Bertolucci, E.; Bisogni, M.G.; Bottigli, U.; Campbell, M.; Chesi, E.; Ciocci, M.A.; Conti, M.; Da Via, C.; Del Guerra, A.; D'Auria, S.; Fantacci, M.E.; Gambaccini, M.; Grossi, G.; Heijen, E.; Mancini, E.; Marchesini, R.; Middelkamp, P.; O'Shea, V.; Randaccio, P.; Romeo, N.; Rosso, V.; Russo, P.; Scharfetter, L.; Smith, K.; Snoeys, W.; Beccherle, R.;

1997 / IEEE


This item was taken from the IEEE Periodical ' Use of silicon and GaAs pixel detectors for digital autoradiography ' Solid state detectors made of Si (4.8/spl times/8 mm/sup 2/) and GaAs (6.4/spl times/8 mm/sup 2/) pixel matrices bump-bonded to the Omega2 and Omega3 electronic read-out systems, developed at CERN for H.E.P. experiments, have been used to obtain autoradiographic images of clusters of human epithelial cells and DNA fragments separated via electrophoresis, both labeled with /sup 32/P. The system has shown a good minimum detectable activity per unit area of 2.10/sup -4/ cps mm/sup -2/, and has proved linear for a count rate in the range 0.2-20 cpa, typical of autoradiography. The pixel dimensions are 75/spl times/500 /spl mu/m/sup 2/ (Si-Omega2) and 50/spl times/500 /spl mu/m/sup 2/ (GaAs-Omega3), respectively. We are able to clearly localize clusters of cells which have incorporated the radioactive tracer and DNA fragments on an electrophoretic gel on paper (blots).